Five of 32 apparently healthy deer, Odocoileus virginianus, were shown to harbor L. monocytogenes in their alimentary tracts. Cultural, biochemical, serologic and virulence characterizations of the five fecal isolates of L. monocytogenes are presented. It is believed that these isolates are the first described from the alimentary tract of apparently normal deer. Two deer harbored serotype I strains of L. monocytogenes, the other three yielded serotype IV strains.