We describe a new species of Anolis from the Andes of northern Peru. This form is similar to species formerly assigned to the genus Phenacosaurus and to Ernest Williams’ tigrinus series. That is, the new species possesses large smooth headscales, cryptic coloration, and short limbs and tail. We present new DNA and morphological data and perform a comprehensive phylogenetic analysis of the Dactyloa clade of Anolis. The new species is estimated to be close to phenotypically similar species from Peru and Venezuela. We revise the taxonomy of Dactyloa based on our estimate. Most previously recognized phylogenetically defined groups are retained with restricted species contents. Several species currently scorable only for morphological data could not be placed in any group with confidence. The species status of the enigmatic mainland form Anolis deltae should be re-examined with reference to the roquet group of southern Lesser Antillean Anolis.