Many attempts have been undertaken to make permanent preparations of helminth eggs. However, the resulting preparations either lacked durability or tended to deform thin-shelled eggs, such as those of the hookworm. To overcome these drawbacks, we have modified 2 aspects of the glycerin-jelly mounting procedure. First, we gradually changed the media in which the helminth eggs soaked, from 10% formalin via water to a 70% ethanol and 5% glycerin solution. It took 10 days, which is much longer than the time required for the processes previously reported. Second, we used a hole slide glass instead of a slide glass. Eggs of 11 species of helminths have been prepared with this procedure, and have kept their morphology without apparent change for more than 4 yr.
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1 April 2010
An Improved Glycerin-Jelly Mounting Procedure for Permanent Preparations of Helminth Eggs
M. Kumagai,
T. Inaba,
A. Makioka,
K. Ishiwata,
K. Onishi,
N. Watanabe
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Journal of Parasitology
Vol. 96 • No. 2
April 2010
Vol. 96 • No. 2
April 2010