Centrifugation is performed on whole blood samples to obtain serum or plasma for biochemical analysis. Although blood samples centrifuged in a microhematocrit tube may maximize recovery of plasma from small-volume samples, plasma biochemical values from such samples have been implicated as causing erroneous results. To compare blood biochemical values obtained by microhematocrit centrifugation and centrifugation with a commercial tilt-rotor machine, blood samples were collected from peregrine falcon (Falco peregrinus) eyases aged 32–40 days (n = 51). The samples were separated into 2 equal aliquots with 1 aliquot centrifuged in a tilt-rotor machine and the other aliquot ultracentrifuged in microhematocrit tubes. Separated plasma from both processes was sent to a commercial veterinary reference laboratory for routine clinical biochemical analysis. No significant differences were found in the biochemical results of the paired samples by the 2 centrifugation methods. These results show that the centrifugation method has no effect on the plasma quality for biochemical analysis in young peregrine falcons.
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1 September 2012
Effects of Ultracentrifugation on Plasma Biochemical Values of Prefledged Wild Peregrine Falcons ( Falco peregrinus) in Northeastern Illinois
Joel Pond,
Steve Thompson,
Mary Hennen,
John Pauley,
Kathryn C. Gamble
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Avian
centrifugation
Falco peregrinus
microhematocrit
Peregrine Falcon
plasma biochemical analysis