Night-time roosting in Meleagris gallopavo (Wild Turkey) is a quotidian activity that minimizes vulnerability to predators and weather. Roost-site selection in managed Pinus palustris (Longleaf Pine) communities is poorly documented. We assessed roost-site selection by comparing use and availability of vegetation types at the individual female Wild Turkey home-range level. We monitored 14 Wild Turkeys from February 2011 to June 2012. The Wild Turkeys did not use vegetation types within the estimated home ranges for roosting in proportion to availability (χ² = 601.696, P < 0.001). Female Wild Turkeys roosted in the upland Longleaf Pine in proportion to availability, selected for lowland hardwood, and avoided upland hardwood patches. We documented that roost-site availability is not likely a limiting factor in managed Longleaf Pine forests.