Recovery of the federally threatened Ambystoma cingulatum (Flatwoods Salamander) will require monitoring of known populations, as well as continued searches for additional populations. In an effort to develop recommendations for maximizing efficiency of future surveys of larval Flatwoods Salamanders, we combined data from surveys conducted between 1990 and 2004 in Florida and Georgia. Analysis of these data revealed variation in the number of larvae captured, survey effort, capture rates, and larval body size among years and months. An average of 16 min or 45 one-m long dipnet sweeps was required to catch each larva. For wetlands surveyed twice in a season, results (i.e., larval presence or assumed absence) were consistent in 74% of consecutive surveys. We make recommendations for conducting future surveys and the implementation of a coordinated research and monitoring program for Flatwoods Salamanders.