155 bryophytes are reported from Frakto Forest, Greece, parts of which consist of old-growth mixed forest with spruce (Picea abies), fir (Abies borisii-regis) and beech (Fagus sylvatica). Many of the species recorded extend into the boreal zone in Europe, and are rare or unknown elsewhere in Greece. Three species, Syzygiella autumnalis, Ulota crispa and Ulota crispula are new to Greece, and there are second or confirmed records for Dicranella subulata, Lewinskya breviseta, Mnium lycopodioides, Seligeria recurvata and Solenostoma obovatum. Attention is drawn to a distinctive forest form of Ctenidium molluscum occurring on non-calcareous substrates.