Methods for the stepwise isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries are described. Both cell types can be isolated in pure culture with high yields. Dogs are a common species used in the study of atherosclerosis and coronary artery disease. Capacity to isolate endothelial cells and smooth muscle cells from individual canine coronary arteries should prove useful in the study of coronary artery disease.
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1 November 2003
A STEPWISE METHOD FOR THE ISOLATION OF ENDOTHELIAL CELLS AND SMOOTH MUSCLE CELLS FROM INDIVIDUAL CANINE CORONARY ARTERIES
MICHAEL K. DAME,
XINWEN YU,
ROSARIO GARRIDO,
WALTER BOBROWSKI,
J. ERIC MCDUFFIE,
HEDWIG S. MURPHY,
MUDHER ALBASSAM,
JAMES VARANI
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In Vitro Cellular & Developmental Biology - Animal
Vol. 39 • No. 10
November 2003
Vol. 39 • No. 10
November 2003
arteriosclerosis
circulation
culture
dog
heart
vasculature
