A new species of Passiflora (P. subg. Decaloba) from Colombia in the Andean region is described and illustrated in this article, using and analysis of 81 morphological descriptors. Passiflora quimbayensis is named in honor to the Pre-Columbian Quimbaya civilization which once lived in the region where the species was discovered. Its closest relative is P. magdalenae, and can be distinguished from it by its peduncle (40–55 mm), sepal (12–15 mm), and androgynophore (5–6.5 mm) lengths, by the number of laminar ocellate-nectaries (6–9, mean 7.5), biseriate corona filaments, globose ovary, and by growing under different ecological conditions (1072 to 1249 m a. s. l.). It is a new endemic species to Colombia and must be regarded as endangered because of its limited occurrence.