The vascular plants of Tennessee's Prentice Cooper State Forest and Wildlife Management Area (PCSFWMA) were gathered from the growing seasons of 1998 through 1999 and the summer of 2005. The ca. 10,300 ha tract of eastern deciduous forest comprises 137 families, 536 genera, and 1,070 species or lesser taxa. Five hundred and eighteen new county records were documented as well as two notable state records, Galium uniflorum and Macrothelypteris torresiana. Nineteen taxa of either state or federal listing were documented or examined. PCSFWMA has 192 introduced species, comprising 17.9% of the total flora and constituting 56.6% of Tennessee's listed invasive exotic pest species. Association coefficients of seven southeastern Appalachian floras were compared to elucidate several floristic similarities and dissimilarities.