CO₂ release rates were measured from groups of 10 Reticulitermes virginicus Banks workers, soldiers, and nymphs. For workers, Vij_co2, (μl·mg⁻¹·h⁻¹) increased linearly with temperature between 16.2 - 30.4°C. Vij_co2 recorded at ≈20°C was 0.177 ± 0.005 μl·mg⁻¹·h⁻¹ for soldiers and 0.219 ± 0.027 (μl·mg⁻¹·h⁻¹ tor nymphs. Assuming a similar slope of temperature increase in Vij_co2 for all castes, predicted mass-corrected CO₂ release values for grouped R. virginicus workers, soldiers and nymphs at 23.6°C were 27, 52 and 27% lower than literature values for the same castes of R. flavipes measured individually at that temperature. CO₂ release rate (μl·mg⁻¹·h⁻¹) for R. virginicus nearly doubled over the 20°C to 30°C temperature range (Q₁₀ = 1.91), similar to literature values for Zootermopsis nevadensis (Hagen) over the same range. For all temperatures except 25.2°C, CO₂ release rate (in μl·h⁻¹) increased significantly with mass, with coefficients ranging from 0.123 (16.2°C) to 0.599 (30.4°C).