Echinops spinosissimus Turra subsp. neumayeri (Vis.) Kožuharov is reported for the first time in Greece, in the peripheral unit of Preveza, close to the Ionian Sea. A closest geographical proximity than previously thought between E. spinosissimus subsp. neumayeri and the other subspecies of E. spinosissimus Turra, subsp. spinosissimus and subsp. bithynicus (Boiss.) Greuter is brought to light. This implies that the eventuality of genetic contacts should be considered when deciphering the evolutionary history of these taxa.