Larvicidal activity and cytotoxicity of fruit and leaf extracts of the Capparis cartilaginea were evaluated. No previous studies have been reported on the larvicidal activities of crude and column chromatographic fractions of fruit and leaf extracts of Capparis cartilaginea. In this study, HPLC analysis of the alkaloidal active fraction F2 of methanolic fruit extract showed the presence of a major peak. Crude aqueous fruit extract of C. cartilaginea was less toxic against Aedes caspius (LC50 = 172.02) compared to its column chromatographic fraction F2 (LC50 = 29.17). Cytotoxicity studies on HepG2 and MCF7 at 24 h post-treatment recorded survival rates ranging from 90 to 110 %, respectively. Light and fluorescent microscopy revealed no effect on cell morphology at 100 µg/ml. The plasma membrane remained well defined and the nuclei were normal and exhibited diffused staining of the chromatin. Most active component(s) responsible for toxic activity against Aedes caspius was present in the fraction F2 of C. cartilaginea, which showed larvicidal efficacy in a dose-dependent manner. As a result, fraction F2 is a good candidate as an alternative natural larvicidal agent with no hepatotoxicity and ata low cost. Further investigation is required to identify and characterize active compounds.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 22 • No. 4