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1 March 2016 Isolation and Functional Characterisation of Antibacterial Peptide from the Mosquito, Aedes (ochlerotatus) caspius (Diptera: Culicidae)
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Abstract

One antimicrobial peptide was isolated and purified to 5.50-fold from the crude lysate of immunised Aedes caspius larvae by (NH4)2SO4 fractionation, ion-exchange chromatography, and reverse-phase high performance liquid chromatography (HPLC). The peptide, named ACAm, was found to be heat stable below 80 °C and exhibited strong antimicrobial activities against Gram-positive and Gram-negative bacteria as well as against peptidoglycans and lipopolysaccharides of bacterial cell walls. The estimated molecular weight of ACAm by sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE), under non-reducing conditions, was 4 kDa. Using isoelectric focusing (IEF), ACAmexhibited pI in basic pH (8.4) belonging to cationic peptides.

©Entomological Society of Southern Africa
G.M. El-Bassiony, W.I.M. Ahmed, M.B.E. Mahmoud, and N.M.M. Elwan "Isolation and Functional Characterisation of Antibacterial Peptide from the Mosquito, Aedes (ochlerotatus) caspius (Diptera: Culicidae)," African Entomology 24(1), 197-207, (1 March 2016). https://doi.org/10.4001/003.024.0197
Accepted: 1 September 2015; Published: 1 March 2016
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