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1 November 2017 Novel Microsatellite Markers for Notopterygium oviforme (Apiaceae), an Endangered Herb Endemic to China
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Abstract
Notopterygium oviforme (Apiaceae) is an endangered perennial herb endemic to China. To investigate its population genetics, we characterized 17 novel microsatellite markers based on high throughput Illumina MiSeq sequencing data. The number of observed alleles (N O) per locus varied between 1 and 7 (mean = 3.53). Observed and expected heterozygosities (H O and H E, respectively) ranged from 0.24 to 0.72, and from 0.37 to 0.64, respectively. Fourteen primers were successfully cross-amplified in two congeneric species (N. incisum and N. forbesii). We detected no deviation from the Hardy-Weinberg equilibrium (HWE) and linkage disequilibrium (LD) at any loci. These microsatellite markers are likely to be useful for the study of population genetic diversity and genetic structure of N. oviforme and its congenerics.
© Finnish Zoological and Botanical Publishing Board 2017
Ying He, Zhe Zhao, Yuan-Yuan Shi, Jiao Yang, Yun Jia, Lin-Lin Gong and Zhong-Hu Li "Novel Microsatellite Markers for Notopterygium oviforme (Apiaceae), an Endangered Herb Endemic to China," Annales Botanici Fennici 54(4–6), (1 November 2017). https://doi.org/10.5735/085.054.0624
Received: 6 June 2017; Accepted: 16 October 2017; Published: 1 November 2017
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