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1 November 2008 Microbial Diversity Associated with the Fruit-Piercing and Blood-Feeding Moth Calyptra thalictri (Lepidoptera: Noctuidae)
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Abstract

Previous inventories of the diversity of lepidopteran symbionts have been limited to Eubacteria. We conducted a microbial survey of Calyptra thalictri Borkhausen (Lepidoptera: Noctuidae) by using polymerase chain reaction (PCR) primers for 16S rRNA sequences for Eubacteria, and primers for Archaea, fungi including yeast-like organisms, Microsporidia, and Wolbachia. Heads and abdomens of adult males of this fruit-piercing and blood-feeding moth were assayed separately. High-fidelity PCR and subsequent DNA analyses indicated that at least five microorganisms belonging to the α-, β-, and γ-Proteobacteria were present. Two eubacterial sequences, related to a Klebsiella sp. and a Sinorhizobium sp., were detected in the abdomens of all nine individuals sampled, and three additional sequences, two related to species in the genus Alcaligenes and one related to a Rhizobium sp., were found in some of the abdominal samples, suggesting all five could be associated with abdominal structures. No Archaea, fungi including yeast-like organisms, Microsporidia, or Wolbachia were detected. These results document the first microbial associates in a fruit-piercing and blood-feeding moth.

Jennifer M. Zaspel and Marjorie A. Hoy "Microbial Diversity Associated with the Fruit-Piercing and Blood-Feeding Moth Calyptra thalictri (Lepidoptera: Noctuidae)," Annals of the Entomological Society of America 101(6), 1050-1055, (1 November 2008). https://doi.org/10.1603/0013-8746-101.6.1050
Received: 14 July 2007; Accepted: 1 September 2008; Published: 1 November 2008
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