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1 May 2009 Mitochondrial DNA Differentiation Between Two Closely Related Species, Phlebotomus (Paraphlebotomus) chabaudi and Phlebotomus (Paraphlebotomus) riouxi (Diptera: Psychodidae), Based on Direct Sequencing and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
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Abstract
Phlebotomus chabaudi Croset, Abonnenc & Rioux, 1970 and Phlebotomus riouxi Depaquit, Killick-Kendrick & Léger, 1998 (Diptera: Psychodidae) are closely related species of phlebotomine sand flies, the females of which are suspected of transmitting Leishmania killicki Rioux, Lanotte, and Pratlong 1986 in Tunisia. Although males of these species are distinguishable by the number and size of setae on the basal lobe of the coxite, morphologically differential characters between the females are blurred. A molecular study, based on 40 Algerian and Tunisian specimens, was conducted to distinguish females of these two species. Alignment of the cytochrome c oxidase I (COI) sequences and their analysis by using neighbor-joining and maximum likelihood showed the separation of P. chabaudi and P. riouxi, irrespective of intraspecific variability. Both COI and cytochrome b have been tested as molecular identification tools. Single strand conformation polymorphism analysis did not distinguish these two species, whereas restriction fragment length polymorphism can be applied to identify P. chabaudi and P. riouxi for surveillance purposes.
© 2009 Entomological Society of America
Raja Boudabous, Azzedine Bounamous, Damien Jouet, Jérôme Depaquit, Denis Augot, Hubert Ferté, Sélima Berchi, Arnaud Couloux, Michel Veuille and Hamouda Babba "Mitochondrial DNA Differentiation Between Two Closely Related Species, Phlebotomus (Paraphlebotomus) chabaudi and Phlebotomus (Paraphlebotomus) riouxi (Diptera: Psychodidae), Based on Direct Sequencing and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism," Annals of the Entomological Society of America 102(3), (1 May 2009). https://doi.org/10.1603/008.102.0301
Received: 1 July 2008; Accepted: 1 October 2008; Published: 1 May 2009
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