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1 July 2010 Molecular Cloning and Expression Pattern of a Lysophospholipase Gene from Antheraea pernyi (Lepidoptera: Saturniidae)
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Abstract

Lysophospholipase (LysoPLA) is one of the most important deacylating phospholipases. The LysoPLA gene from Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae) (ApLysoPLA), a well-known wild silkmoth, was isolated and characterized. The isolated ApLysoPLA cDNA sequence was 1151 bp, with an open reading frame of 663 bp encoding a polypeptide of 220 amino acids. The deduced ApLysoPLA protein sequence has 89 and 82% identity with Heliconius reato (L.) LysoPLA and Bombyx mori L. LysoPLA, respectively; however, it shows 66, 62, 50, and 47% identity with Tribolium castaneum (Herbst) LysoPLA, Drosophila melanogaster (Meigen) LysoPLA, Homo sapiens LysoPLA I, and Mus musculus LysoPLA I, respectively. Phylogenetic analysis indicated that lepidopteran LysoPLAs, including ApLysoPLA, might be a new member of the LysoPLA family. Semiquantitative reverse transcription-polymerase chain reaction analysis showed that the ApLysoPLA gene was transcribed during four developmental stages (egg, larva, pupa, and adult) and that it was present in all tissues tested (blood, midgut, silk glands, Malpighian tubules, spermaries, ovaries, brain, muscle, fat body, and body wall), with the most abundance in Malpighian tubules. Consequently, we refer that ApLysoPLA plays an important role in the development of A. pernyi.

© 2010 Entomological Society of America
Yan-Qun Liu, Yu-Ping Li, Song Wu, Run-Xi Xia, Sheng-Lin Shi, Li Qin, Cheng Lu, and Zhong-Huai Xiang "Molecular Cloning and Expression Pattern of a Lysophospholipase Gene from Antheraea pernyi (Lepidoptera: Saturniidae)," Annals of the Entomological Society of America 103(4), (1 July 2010). https://doi.org/10.1603/AN09151
Received: 18 October 2009; Accepted: 16 March 2010; Published: 1 July 2010
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