Translator Disclaimer
1 November 2011 Molecular Cloning, Expression Pattern, and Phylogenetic Analysis of a Selenophosphate synthetase Gene from Antheraea pernyi (Lepidoptera: Saturniidae)
Author Affiliations +
Abstract
Selenophosphate synthetase (Sps), the product of the SelD gene, produces a biologically active selenium donor compound from ATP and selenide. We have isolated and characterized the Sps gene from Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae), an economically important insect. The resulting 1601 bp cDNA sequence contains an open reading frame of 1209 bp encoding a polypeptide of 402 amino acids, with 87% sequence identity to that from Drosophila melanogaster (Meigen). Semiquantitative reverse transcription-polymerase chain reaction (PCR) analysis showed that the Sps gene was transcribed during four developmental stages (egg, larva, pupa, and adult) and in all the tissues tested (blood, fat body, midgut, silk glands, body wall, spermaries and ovaries), suggesting that ApSps plays an important role in the development of A. pernyi. From a database search, Sps protein homologs were found in prokaryotes and eukaryotes, including bacteria, fungi, invertebrates and vertebrates, with 47–98% amino acid sequence identities between eukaryotes, suggesting that they were highly conserved during the evolution of eukaryotes. Phylogenetic analysis, based on Sps protein homolog sequences, clearly separated the known bacterial, fungal, invertebrate and vertebrate Sps proteins, consistent with the topology tree of classical systematics, suggesting the potential value of the Sps protein sequence in phylogenetic inference.
© 2011 Entomological Society of America
Shou-Hui Sun, Yu-Ping Li, Ya-Nan Zheng, Xiao-Rui Xu, Xiang-Dong Jin, Song Wu, Wen-Li Li and Yan-Qun Liu "Molecular Cloning, Expression Pattern, and Phylogenetic Analysis of a Selenophosphate synthetase Gene from Antheraea pernyi (Lepidoptera: Saturniidae)," Annals of the Entomological Society of America 104(6), (1 November 2011). https://doi.org/10.1603/AN11068
Received: 8 April 2011; Accepted: 1 July 2011; Published: 1 November 2011
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top