Within an anonymous region of honey bee DNA (locus 227) digested with AluI, informative restriction fragment-length polymorphisms (RFLP) were found in Southern blots with a cloned honey bee DNA probe. The probe was subcloned, so that smaller sections of the locus could be analyzed with the polymerase chain reaction (PCR). Further screening of these amplified sections revealed additional useful RFLPs with HinfI. The informative AluI and HinfI polymorphic sites were mapped to a narrow section of the original probe. A total of 14 sub-alleles was found in this region of which five were found only or predominantly in our African samples (Apis mellifera scutellata Lepeletier), three were found only or predominantly in our east European samples (A. m. ligustica Spinola’ A. m. carnica Pollman, and A. m. caucasica Gorbachev), one was found predominantly in our west European samples (A. m. mellifera L. and A. m. iberica Goetze). Significant associations were found between the AluI and HinfI sub-alleles, reinforcing their subspecies group specificity.
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Vol. 95 • No. 1