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1 May 2002 Wolbachia Infection in the Coffee Berry Borer (Coleoptera: Scolytidae)
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Abstract

A nested polymerase chain reaction protocol yielded positive detection of the maternally inherited cytoplasmic proteobacterium Wolbachia in total genomic DNA from coffee berry borers collected in Benin, Brazil, Colombia, Ecuador, El Salvador, Honduras, India, Kenya, Mexico, Nicaragua, and Uganda. Wolbachia was not detected in specimens from Cameroon, the Dominican Republic, Indonesia, Jamaica, and Peru. Amplified bands from India and Brazil were cloned and sequenced. The 438-bp sequence clearly conformed to Wolbachia group B and was nearly identical to that of Ephestia kuehniella. The possible implications of Wolbachia infection in the coffee berry borer are discussed.

Fernando E. Vega, Pablo Benavides, Jeffrey A. Stuart, and Scott L. O’Neill "Wolbachia Infection in the Coffee Berry Borer (Coleoptera: Scolytidae)," Annals of the Entomological Society of America 95(3), 374-378, (1 May 2002). https://doi.org/10.1603/0013-8746(2002)095[0374:WIITCB]2.0.CO;2
Received: 24 August 2001; Accepted: 1 February 2002; Published: 1 May 2002
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