Translator Disclaimer
1 July 2006 Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease
Author Affiliations +
Abstract

Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of human Plasmodium in parts of Brazil. We report the first record of An. marajoara in the department of Cochabamba and confirm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon (≈340 bp) of a section of the internal transcribed spacer 2 specific for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia.

C. L. Brelsfoard, G. N. Fritz, and R. Rodriguez "Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease," Annals of the Entomological Society of America 99(4), 707-713, (1 July 2006). https://doi.org/10.1603/0013-8746(2006)99[707:IOANMD]2.0.CO;2
Received: 4 September 2005; Accepted: 1 March 2006; Published: 1 July 2006
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top