Spiders from the steppe habitats of the Pláně Nature Monument (Czech Republic) were investigated. The spiders were samdoi: 10.30963/aramit5812 pled using pitfall traps and through sweeping of the herb vegetation. In total, 11634 individuals from 154 species in 88 genera from 25 families were recorded. Numerous threatened species listed in the Red List of the Czech spiders were found (CR – 1, EN – 1, VU – 11, LC – 23). 36 mainly xerothermic species were recorded, representing a valuable arachnofauna typical for the Pannonian Region. However, the studied area does not belong to this particular region. Several findings represent the northernmost occurrences of rare thermophilic spiders in the Czech Republic. Ten species (Dysdera moravica, Megalepthyphantes pseudocollinus, Trichoncus affinis, Porrhomma errans, Alopecosa striatipes, Cheiracanthium oncognathum, Civizelotes pygmaeus, Zelotes aeneus, Thanatus arenarius, Talavera aperta) are discussed in details and their records in the Czech Republic are mapped. Suggestions regarding the management of the area are given to improve the conditions for a high and valuable biodiversity.