High mountain environments are highly stressful for insect survival. It has been suggested that small microtopographic variations generating less stressful microclimatic conditions than the surrounding environment would provide more suitable sites for insect development. Cushion plants represent one of the life forms best adapted to the extreme alpine habitats. Cushion plants can modify microclimatic conditions within and under their canopy, generating less severe microsites than the surrounding environment. In this study, we characterized the microclimatic modifications made by the cushion plants Azorella monantha and Laretia acaulis and examine their role as microclimatic shelters for two species of high Andean coleopterans (Coccinelidae): Eriopis connexa and Hippodamia variegata at 3200 m a.s.l. in the Andes of central Chile. Results showed that the cushion species create microhabitats with higher availability of water and less oscillating temperatures. However, the intensity of modifications was higher in A. monantha compared to L. acaulis. The abundance of the two ladybird beetle species was higher within cushions than outside, although E. connexa showed higher abundances compared to H. variegata. However, a habitat selection experiment in a greenhouse showed that under milder temperature conditions ladybird beetles species do not prefer cushions. This suggests that in the harsh alpine climate, cushion plants may act as microclimatic shelters since they reduce stressful environmental conditions, allowing greater abundances of coleopterans than in the surrounding environment.
High mountain environments are very stressful habitats for insect survival. The main climatic characteristics of these habitats are extreme temperatures, strong winds, nutrient and water shortage, and short growing seasons (Körner, 1999, 2000). Extreme temperatures and daily high thermal oscillation are the main factors determining insect reproduction success and survival (Matthews and Kitching, 1984; Jones et al., 2001). Other factors determining successful colonization by high-mountain insects are soil water availability and wind speed, since these factors affect oviposition rates and heat loss through evapotranspiration (Rockstein, 1964; Matthews and Kitching, 1984; Laub and Luna, 1992). It has been widely observed that many insects occurring in extreme environments are closely associated with plants, thus reducing the severe environmental conditions (Manley, 1996; Jones et al., 2001). Indeed, Matthews and Kitching (1984) and Manley (1996) have suggested that small microtopographic variations generate less stressful microclimatic conditions, which provide more suitable sites for insect development.
Cushion plants are one of the best-adapted growth forms in high mountain environments (Pysek and Lyska, 1991; Körner, 1999). Their small stature and compact architecture allow them to generate less severe microclimate conditions compared to their surrounding environment, mitigating unfavorable environmental effects (Cavieres et al., 1998; Körner, 1999). Microclimates created by cushion plants reduce extreme temperatures and enhance both water and nutrient availability (Cavieres et al., 1998; Nuñez et al., 1999; Körner, 1999). Considering that high mountain regions present very limited opportunities for insect development, and that cushion plants provide less stressful microclimatic conditions, it would be expected that the latter serve as microclimatic refuges for insects. We have observed that at least two ladybird beetles species inhabit the alpine zone of central Chile, with those species being observed more frequently within cushion plants (Fig. 1). In order to test the hypothesis that cushions represent a shelter for insect species, we compared the abundance and richness of ladybird beetle species distributed on two cushion plants, Azorella monantha Clos (Apiaceae) and Laretia acaulis (Cav.) Gill. et Hook (Apiaceae), as well as in surrounding open areas, at 3200 m a.s.l. in the alpine zone of central Chile.
Materials and Methods
This study was conducted in the Andes of central Chile near the locality of Valle Nevado (33°20′S, 70°16′W), 50 km east of the city of Santiago. Climate is typically alpine, with strong influence of the Mediterranean-type climate that prevails in lowlands (di Castri and Hajek, 1976). In winter, mean annual air temperature is 1.7°C, with an absolute minimum of −15.0°C (Cavieres and Arroyo, 1999). In summer, mean annual air temperature is 6.8°C, and the absolute maximum is 17.0°C (Cavieres and Arroyo, 1999). Mean annual precipitation ranges from 500–900 m, occurring mainly as snow during winter months (Santibañez and Uribe, 1990).
At 3200 m elevation, on an east-facing slope, we selected an area of approximately 20,000 m2, where vegetation was dominated by cushions of Azorella monantha and Laretia acaulis.
TEMPERATURE AND HUMIDITY
To characterize microclimate within cushions of Azorella monantha and Laretia acaulis and their surrounding environment, we measured substrate temperature and water availability on two consecutive sunny days in February 2003.
We randomly selected five cushions of each species, and five points in the surrounding environment. At each of these cushions and points in the bare ground, we measured substrate temperature at 1 cm depth with a digital thermometer (Digital Thermometer 871A, Tegam, HO, U.S.A.). Measurements were taken every 2 h between 08:00 and 20:00 h, and registrations on the two microhabitats (cushions and surrounding environment) were made simultaneously in order to obtain comparable data, which were analyzed with repeated measures using ANOVA.
To characterize water availability, soil matric water potential was measured with tensiometers (2725 Series Jet Fill, Soil Moisture, CO, U.S.A.) both beneath cushion plants and in surrounding open areas. These measurements were made at a depth of 10 cm on five cushions of A. monantha, five cushions of L. acaulis, and five random points in the surrounding environment, 2 m away from the edge of any cushion. Measurements within cushions and surrounding open areas were made simultaneously. These data were compared among the three microsites with one-way ANOVA, and a posteriori Tukey tests were used to assess differences between microsites.
RICHNESS AND ABUNDANCE
At the study site, 50 individuals of each cushion species were randomly selected. Previously, we constructed metallic-hoops of known diameter (20, 30, and 40 cm diameter). At each selected cushion, a metallic hoop of similar size was placed over the cushion, and all individual ladybird beetles within the hoop were identified and counted. Sampling was conducted simultaneously in cushions and in the surrounding environment. For this, the metallic hoop used to sample each cushion was placed in the surrounding environment at a double diameter distance, and in a random direction, from the center of the respective cushion. Abundances of beetles (individuals dm−2) were compared among microsites (Azorella monantha, Laretia acaulis, and the surrounding environment) and beetle species with two-way ANOVA. Differences between mean abundances were assessed with a posteriori with Tukey tests.
EXPERIMENT OF HABITAT SELECTION
To test whether cushion plants serve as microclimatic shelters for high-Andean ladybird beetles, 30 individuals of each beetle species detected in field were collected alive and maintained in glass jars. Additionally, three individual cushions of Azorella monantha and other three of Laretia acaulis (40–50 cm diameter) were taken to a greenhouse with controlled conditions of temperature and relative humidity of air (25°C, 75% RH). Three further plots (40 cm diameter, 10 cm depth) filled with soil from open areas surrounding cushions were placed in the greenhouse. In the greenhouse, both cushion plants and plots with soil were disposed on a white cloth sheet following a circular arrangement (2 m diameter). In this circular arrangement, cushion species and plots with soil were regularly alternated. At the center of this system, we placed 20 individuals of each beetle species collected in field and set a photoperiod 16/8 h day-night. After 48 h, we examined cushion plants and plots with soil looking for ladybird beetles. All individuals beetles detected at each experimental unit were identified and counted. These data were compared among microsites and beetle species with two-way ANOVA, and Tukey tests were used to assess differences a posteriori.
TEMPERATURE AND HUMIDITY
Averaging substrate temperatures at each hour, maximum substrate temperatures within both cushion species, as well as in the surrounding environment, were recorded between 12:00 and 16:00 h, whereas minimum temperatures were detected at 8:00 and 20:00 h (Fig. 2). Repeated measures with ANOVA indicated differences in substrate temperatures between microsites (F2,6 = 99.51; P < 0.001) as well as across time (F6,36 = 757.23; P < 0.001). Most of the time, both cushion species maintained lower mean temperatures than the surrounding environment, but cushions of Azorella monantha maintained higher temperatures than Laretia acaulis and open areas at 8:00 and 20:00 h (Fig. 2).
Soil matric potentials within A. monantha and L. acaulis were higher than in the surrounding environment (F2,11 = 663.51; P < 0.001), indicating that both cushion species contain higher soil moisture than surrounding open areas (Fig. 3). Differences between cushion species were also found; A. monantha had higher soil moisture than L. acaulis (Fig. 3).
RICHNESS AND ABUNDANCE
Only two ladybird beetle species were recorded in the study site, Eriopis connexa and Hippodamia variegata. Irrespective of species, a total of 250 individual beetles were detected during sampling, where 86% were found on cushions of Azorella monantha, 13% on cushions of Laretia acaulis, and 1% in the surrounding environment. Of a total of 182 individuals of E. connexa, 82% were found on A. monantha, 17% on L. acaulis, and 1% in the surrounding environment. On the other hand, of a total of 68 individuals of H. variegata, 96% were detected on A. monantha and 4% on L. acaulis. No H. variegata individuals were recorded in the surrounding environment.
Results of the two-way ANOVA indicated that beetle abundances differed significantly between microsites (F2,12 = 2858.9; P < 0.001), as well as between beetle species (F1,12 = 772.6; P < 0.001). Analyses a posteriori indicated that A. monantha was the microsite with the higher abundance of beetles, followed by L. acaulis and the surrounding environment, respectively (Fig. 4). Additionally, these analyses indicated that E. connexa was significantly more abundant that H. variegata in all microsites (Fig. 4).
EXPERIMENT OF HABITAT SELECTION
Since both, Eriopis connexa and Hippodamia variegata were the only beetle species detected in the study site, these ladybird beetles were used to conduct the greenhouse experiment to assess differences in their habitat preferences. Results of this experiment showed no differences in the number of individual beetles neither among microsites (F2,12 = 0.25; P = 0.78) nor between beetle species (F1,12 = 0.11; P = 0.98) (Fig. 5).
Physiological adaptations and the use of microclimatic shelters by insects as a survival strategy in high-mountain environments have been observed worldwide (Schmoller, 1970; Matthews and Kitching, 1984; Somme, 1993; Jones et al., 2001). According to our results, in the high Andes of central Chile, cushions of Azorella monantha and Laretia acaulis may provide microhabitats that could act as thermal refuges, maintaining milder temperatures when the surrounding environment reaches either extremely low or extremely high temperatures, as well as reduce daily thermal oscillation (see Fig. 2). Furthermore, cushion plants maintain higher soil moisture than their surrounding environment, suggesting that both A. monantha and L. acaulis provide habitat patches in which beetle species can have conditions more suitable for survival and development of different life stages in their life cycle.
For example, Acar et al. (2004) and Simmons and Legaspi (2004) have shown that high temperatures (>35°C) decrease the survival both of larval and adults in Coccinellidae species. In our study, while soil in open areas surrounding cushions can easily reach that temperature at midday, cushions never exceeded 30°C (Fig. 2). Regarding soil moisture, Fujiyama et al. (2003) showed that adults of Coccinellidae species are more abundant in soils with high moisture. This, added to the lower larval mortality suggested by Venette et al. (2000) for habitat with low oscillating thermal and hydric conditions, could explain the observed patterns reported here for two ladybird beetle species. Hence, the microclimatic modifications induced by cushion plants could generate microsites more favorable for oviposition, egg development, larval and adult survival, and permanence of Eriopis connexa and Hippodamia variegata in the Andes of central Chile.
The lower beetle abundance recorded in areas surrounding cushions suggest that this microenvironment could be highly stressful for development and survival of the ladybird beetle species inhabiting the high Andes. Conversely, the higher beetle abundance detected within cushion plants reinforces the suggestion that habitat patches created by cushions may act as microclimatic shelters for those beetle species. Nevertheless, abundances of both beetle species detected in the study site (E. connexa and H. variegata) differed between the two cushion species. Such differences may be related to different effects of A. monantha and L. acaulis on microclimatic conditions. For instance, our data indicate that A. monantha mitigates temperature and soil moisture conditions with higher intensity than L. acaulis (see Figs. 1 and 2). Thus, such differences could help to explain why both E. connexa and H. variegata had higher abundances on the former cushion species. This follows the suggestions of Rockstein (1964) and Laub and Luna, (1992), who have found that there is a positive correlation between insects' density and soil hydric content, which could also explain the greater number of individuals of ladybird beetle species on A. monantha than on L acaulis, and on both cushions than in the surrounding environment.
Nevertheless, the distribution of ladybird beetle species among microhabitats could be determined by differences in the availability of prey (e.g., mites, aphids). However, mites of the genera Pheriolodes and Novonthrus were found within both cushion species as well as in soil from the surrounding environment (Torres, data unpublished), suggesting that the observed pattern is unlikely to be related only with differential availability of prey.
Notwithstanding, patterns of beetle abundances obtained in the greenhouse experiment contrasted with those patterns observed in field. In this experiment, ladybird beetle abundance showed no differences between cushions species or between cushions and soil from surrounding environment. This lack of response to the presence of cushion plants might eventually indicate that, under greenhouse-controlled conditions, microclimatic differences between cushions and soil from surrounding environment were reduced, so that distribution patterns of ladybird beetles disappeared.
As occur with many other taxa, the diversity and abundance of Coleoptera fauna in high mountain areas decrease with elevation (McCoy, 1990; Lobo and Halffter, 2000). Nevertheless, the presence of more benign microhabitats (e.g., cushion plants) may extend the altitudinal limits of certain species, maintaining greater diversity and abundance in alpine environments than would be expected without the presence of this shelter. Hence, cushion plants can be considered key elements for the maintenance of diversity of alpine habitats of the central Chilean Andes.
To W. Gonzáles and E. Gianoli for their helpful suggestions in earlier drafts. P. Sommariva-Montenegro for his help during the greenhouse experiment, and V. Jerez and R. Briones for comments and identification of beetle species. O. Buchner and S. Elias are also acknowledged for their valuable criticisms. MAM-M holds a CONICYT Doctoral Fellowship. EIB holds a Doctoral Fellowship from grant MECESUP UCO-9906. This work was funded by the project FONDECYT 1030821. This paper forms part of the research activities of the Millennium Center for Advanced Studies in Ecology and Research on Biodiversity supported by grant no. P02-051-F ICM.
- E. B. Acar, D. D. Mill, B. N. Smith, L. D. Hansen, and G. M. Booth . 2004. Calorespirometric determination of the effects of temperature on metabolism of Harmonia axyridis (Col: Coccinellidae) from second instars to adults. Environmental Entomology 33:832–838. Google Scholar
- L. A. Cavieres and M. T K. Arroyo . 1999. Tasa de enfriamiento adiabático del aire en el valle del río Molina, provincia de Santiago, Chile central (33°S). Revista Geográfica de Chile Terra Australis 44:79–86. Google Scholar
- L. A. Cavieres, A. Peñalosa, C. Papic, and M. Tambutti . 1998. Efecto nodriza del cojín Laretia acaulis (Umbelliferae) en la zona alto-andina de Chile central. Revista Chilena de Historia Natural 71:337–347. Google Scholar
- F. di Castri and E. Hajek . 1976. Bioclimatología de Chile. Santiago, Chile: Ediciones de la Pontificia Universidad Católica de Chile. Google Scholar
- N. Fujiyama, T. Koizumi, and H. Katakura . 2003. Conspecific thistle plant selection by a herbivorous ladybird beetle, Epilachna pustulosa. Entomologia Experimentalis et Applicata 108:33–42. Google Scholar
- H. G. Jones, J. W. Pomeroy, D. A. Walker, and R. W. Hoham . 2001. Snow Ecology. Cambridge: Cambridge University Press. Google Scholar
- C. H. Körner 1999. Alpine Plant Life. Berlin: Springer. Google Scholar
- C. H. Körner 2000. The Alpine life zone under global change. Gayana Botánica 57:1–17. Google Scholar
- C. A. Laub and J. M. Luna . 1992. Winter cover crop suppression practices and natural enemies of armyworm (Lepidoptera: Noctuidae) in no-till corn. Environmental Entomology 12:41–49. Google Scholar
- J. M. Lobo and G. Halffter . 2000. Biogeographical and ecological factors affecting the altitudinal variation of mountainous communities of coprophagous beetles (Coleoptera: Scarabaeoidea): a comparative study. Annals of the Entomological Society of America 93:115–126. Google Scholar
- G. Manley 1996. Relationship between selected intercropped ground covers and soil arthropod populations in a no-till crop rotation system in western St. Joseph County, Michigan (year one, 1995 corn). In Proceedings, Cover Crop Symposium, Michigan State University, East Lansing. Google Scholar
- E. Matthews and R. Kitching . 1984. Insect Ecology. Brisbane: University of Queensland Press. Google Scholar
- E. D. McCoy 1990. The distribution of insects along elevational gradients. Oikos 58:313–322. Google Scholar
- C. Nuñez, M. Aizen, and C. Escurra . 1999. Species associations and nurse plant effect in patches of high-Andean vegetation. Journal of Vegetation Science 10:357–364. Google Scholar
- P. Pysek and J. Lyska . 1991. Colonization of Sibbaldia tetrandra cushions on alpine scree in the Palmiro-Alai mountains, Central Asia. Arctic and Alpine Research 23:263–272. Google Scholar
- M. Rockstein 1964. The Physiology of Insecta. University of Miami School of Medicine, Coral Gables, Florida. Academic Press. Google Scholar
- F. Santibañez and J. M. Uribe . 1990. Atlas agroclimático de la V Región y Región Metropolitana. Santiago: Universidad de Chile. Google Scholar
- R. Schmoller 1970. Life histories of alpine tundra Arachnida in Colorado. American Midland Naturalist 127:66–76. Google Scholar
- A. Simmons and J. Legaspi . 2004. Survival and predation of Delphastus catalinae (Coleoptera: Coccinellidae), a predator of whiteflies (Homoptera: Aleyrodidae), after exposure to a range of constant temperatures. Environmental Entomology 33:839–843. Google Scholar
- L. Somme 1993. Living in the cold. Biologist 40:14–17. Google Scholar
- R. C. Venette, S. E. Naranjo, and W. D. Hutchinson . 2000. Implications of larval mortality a low temperatures and high soil moistures for establishment of pink bolworm (Lepidoptera: Gelechiidae) in southeastern United States cotton. Environment Entomology 29:1018–1026. Google Scholar