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1 September 2015 Characterisation of 14 microsatellite markers for the Australian fig psylloid, Mycopsylla fici
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Abstract

The Australian fig psylloid, Mycopsylla fici, is a sap-feeding insect herbivore that is host-specific to the Moreton Bay fig, Ficus macrophylla. It has periodic major outbreaks that can cause complete defoliation of individual trees and massive decrease in local leaf and fruit availability, with significant effects for many insect and vertebrate species that utilise the tree’s resources. We used ⅛ of an Illumina MiSeq run to sequence genomic DNA from two pools of five psylloids from two different field sites. We identified 14 polymorphic microsatellite loci and characterised them in 43 individuals from two populations (Sydney and Lord Howe Island, Australia). Within populations, the number of alleles ranged from 4 to 15 per locus with observed heterozygosity of 0–0.9. Four loci deviated from Hardy–Weinberg equilibrium. The microsatellite primers will be useful for the study of population genetics and gene flow within and between psylloid populations.

© CSIRO 2015
Caroline Fromont, Markus Riegler, and James M. Cook "Characterisation of 14 microsatellite markers for the Australian fig psylloid, Mycopsylla fici," Australian Journal of Zoology 63(4), 233-235, (1 September 2015). https://doi.org/10.1071/ZO15027
Received: 28 May 2015; Accepted: 1 July 2015; Published: 1 September 2015
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