Translator Disclaimer
1 January 2002 Serologic Detection of Experimental Salmonella enteritidis Infections in Laying Hens by Fluorescence Polarization and Enzyme Immunoassay
Author Affiliations +
Abstract

Detection of infected poultry flocks is essential for controlling eggborne transmission of Salmonella enteritidis to humans. The present study evaluated the detection of antibodies in the sera of experimentally infected chickens by a fluorescence polarization assay with a tracer prepared from the O-polysaccharide of S. enteritidis and an enzyme-linked immunosorbent assay (ELISA) with an S. enteritidis flagellin antigen. In two trials, groups of specific-pathogen-free laying hens were infected orally with either 106 or 108 colony-forming units (CFU) of S. enteritidis (phage type 13a) or with 108 CFU of Salmonella typhimurium. Serum samples were collected before inoculation and at five subsequent weekly intervals. Both assays successfully detected the majority of hens infected with S. enteritidis at either dose level, but they also identified a substantial number of hens infected with S. typhimurium as seropositive. The fluorescence polarization test detected S. enteritidis infection significantly more often and cross-reacted with sera from hens infected with S. typhimurium significantly less often than the ELISA. The fluorescence polarization assay also offered advantages in terms of speed and methodologic simplicity.

Richard K. Gast, Mohammad S. Nasir, Michael E. Jolley, Peter S. Holt, and Henry D. Stone "Serologic Detection of Experimental Salmonella enteritidis Infections in Laying Hens by Fluorescence Polarization and Enzyme Immunoassay," Avian Diseases 46(1), 137-142, (1 January 2002). https://doi.org/10.1637/0005-2086(2002)046[0137:SDOESE]2.0.CO;2
Received: 26 June 2001; Published: 1 January 2002
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top