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1 April 2003 Isolation and Characterization of a 6/85-like Mycoplasma gallisepticum from Commercial Laying Hens
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Abstract

Eighty-three-week-old table egg layers with swollen sinuses were presented with a history of increased mortality. Serology revealed positive titers to Mycoplasma gallisepticum (MG). The birds were part of a flock in which some birds had been vaccinated with 6/85 live MG vaccine at 18 wk of age. Tracheal cultures were obtained from both vaccinated and unvaccinated birds within the flock. The cultures were indistinguishable from 6/85 vaccine by both random amplified polymorphic DNA analysis and DNA sequence analysis. Challenge studies were performed to compare the field isolates with 6/85 vaccine and the R strain of MG. The field isolates produced a greater antibody response by serum plate agglutination than did the 6/85 vaccine. The isolates effectively colonized the trachea without increasing the tracheal mucosal thickness; however, they did not extensively colonize the air sacs or cause airsacculitis in the experimental birds.

S. J. Throne Steinlage, N. Ferguson, J. E. Sander, M. García, S. Subramanian, V. A. Leiting, and S. H. Kleven "Isolation and Characterization of a 6/85-like Mycoplasma gallisepticum from Commercial Laying Hens," Avian Diseases 47(2), (1 April 2003). https://doi.org/10.1637/0005-2086(2003)047[0499:IACOAL]2.0.CO;2
Received: 15 October 2002; Published: 1 April 2003
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