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1 September 2003 Development and Preliminary Validation of an Ad Hoc N1-N3 Discriminatory Test for the Control of Avian Influenza in Italy
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Abstract
The development of a discriminatory test, based on the differentiation between N1 and N3 antibodies, to be used in the framework of a vaccination program, based on vaccination with a heterologous H7N3 inactivated vaccine against the Italian H7N1 field virus, is reported. The indirect immunofluorescence antibody (iIFA) assay was based on the expression of the N1 protein in a baculovirus system. HighFive® insect cells were transfected with the recombinant virus and used as an antigen in the iIFA test. Preliminary validation on 608 turkey sera yielded relative sensitivity and specificity of 98.1% and 95.7%, respectively, when compared to the HI test with an almost perfect agreement between the two methods (Kappa value = 0.93). It is concluded that the iIFA test is a valid tool for monitoring avian influenza infection in a vaccinated population.
G. Cattoli, C. Terregino, V. Brasola, J. F. Rodriguez and I. Capua "Development and Preliminary Validation of an Ad Hoc N1-N3 Discriminatory Test for the Control of Avian Influenza in Italy," Avian Diseases 47(s3), (1 September 2003). https://doi.org/10.1637/0005-2086-47.s3.1060
Received: 14 April 2002; Accepted: ; Published: 1 September 2003
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