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1 September 2003 A NASBA Method to Detect High- and Low-Pathogenicity H5 Avian Influenza Viruses
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Abstract

Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.

R. A. Collins, L. S. Ko, K. L. So, T. Ellis, L. T. Lau, and A. C. Hoi Yu "A NASBA Method to Detect High- and Low-Pathogenicity H5 Avian Influenza Viruses," Avian Diseases 47(s3), 1069-1074, (1 September 2003). https://doi.org/10.1637/0005-2086-47.s3.1069
Received: 14 April 2002; Published: 1 September 2003
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