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1 September 2003 RT-PCR-ELISA as a Tool for Diagnosis of Low-Pathogenicity Avian Influenza
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Abstract

A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected with low-pathogenicity AIV, from wild aquatic birds, and from domestic ducks. The AIV was detected in 32 swab pools by RT-PCR-ELISA compared to 23 by virus isolation (VI) in embryonated specific pathogen free (SPF) chicken eggs. Thus, 39% more specimens were positive by RT-PCR-ELISA than by VI. Two of the twenty-three VI-positive specimens were negative when tested by RT-PCR-ELISA. The diagnostic sensitivity and specificity of the RT-PCR-ELISA was 91% and 97%, respectively, using VI in SPF eggs as the gold reference standard.

Karen Dybkær, Mette Munch, Kurt J. Handberg, and Poul H. Jørgensen "RT-PCR-ELISA as a Tool for Diagnosis of Low-Pathogenicity Avian Influenza," Avian Diseases 47(s3), 1075-1078, (1 September 2003). https://doi.org/10.1637/0005-2086-47.s3.1075
Received: 14 April 2002; Published: 1 September 2003
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