Translator Disclaimer
1 September 2003 Development of a Highly Sensitive Screen for Influenza A in Guano and Its Application in the Search for Ancient RNA Preserved Under Antarctic Adelie Penguin Colonies
Author Affiliations +
Abstract

We have developed a reverse transcriptase polymerase chain reaction (RT-PCR)-based assay to detect influenza A in guano samples as part of our program to investigate ancient viral RNA from under Antarctic Adelie penguin (Pygoscelis adeliae) colonies. Of five extraction protocols tested (RNeasy, GTC TRIZOL, GTC Silica, Rnaid, and AGPC), AGPC proved to be the most consistent and sensitive to low concentrations of influenza, but further purification with commercial viral nucleic acid spin filter system was still required to remove remaining PCR inhibitors. RT-PCR was then performed on the eluent and 650 bases of the M1 gene were amplified. The assay was found to be able to detect as little as 100 μl of 0.1 hemagglutination units (HU)/ml influenza.

L. C. Briggs, R. M. Ashton, and P. Metcalf "Development of a Highly Sensitive Screen for Influenza A in Guano and Its Application in the Search for Ancient RNA Preserved Under Antarctic Adelie Penguin Colonies," Avian Diseases 47(s3), 1200-1202, (1 September 2003). https://doi.org/10.1637/0005-2086-47.s3.1200
Received: 14 April 2002; Published: 1 September 2003
JOURNAL ARTICLE
3 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top