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1 September 2005 Development of Quantitative Real-Time Polymerase Chain Reaction for Duck Enteritis Virus DNA
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Abstract

Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, we introduce a quantitative real-time polymerase chain reaction (PCR) assay for DEV DNA using TaqMan technology and a two-step protocol. It was confirmed to be rapid, sensitive, and specific for DEV detection. The primers and probe were designed and directed to the DNA polymerase gene of DEV. The method will provide a valuable tool for rapid laboratory diagnosis of DEV infection. By virtue of its high-throughput format and its ability to accurately quantify the viral DNA, the method may be useful for large epidemiological surveys and clarification of pathogenesis, such as latency and reactivation of the virus.

Fa-Long Yang, Wen-Xiang Jia, Hua Yue, Wei Luo, Xi Chen, Yi Xie, Wei Zen, and Wei-Qin Yang "Development of Quantitative Real-Time Polymerase Chain Reaction for Duck Enteritis Virus DNA," Avian Diseases 49(3), 397-400, (1 September 2005). https://doi.org/10.1637/7338-020305R.1
Received: 3 February 2005; Accepted: 1 May 2005; Published: 1 September 2005
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