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1 September 2008 Prevalence of Tetracycline-Resistant Campylobacter in Organic Broilers During a Production Cycle
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Abstract

Tetracycline (tet) resistance in Campylobacter isolated from organically raised broilers was investigated in this study. Two hundred forty-five samples from an organic broiler farm were collected weekly from the first week to the end of the production cycle, and they were cultured for thermophilic Campylobacter. Tetracycline resistance of these Campylobacter isolates was identified by the agar dilution method, whereas DNA fingerprinting profiles of tet-susceptible and tet-resistant strains were determined by pulsed-field gel electrophoresis (PFGE). None of the Campylobacter isolates from the third and the fourth week of the production period were resistant to tetracycline, whereas 66.7% of the isolates from the fifth week were resistant to this antibiotic. Although the prevalence of tetracycline resistance reached 100.0% during the sixth and seventh week, less than 34.0% of the isolates from the 10th week were resistant to this antimicrobial agent. In addition, only 13.8% of Campylobacter isolates from the intestinal tracts of these organically raised broilers were resistant to tetracycline. The presence of the tet(O) gene was detected in 98.9% of tet-resistant Campylobacter isolates, and tet-susceptible and tet-resistant Campylobacter strains showed distinct PFGE genotypes. The results suggest that the Campylobacter strains isolated from the early stage of the production were susceptible to tetracycline, but they were subsequently displaced by tet-resistant Campylobacter.

Nota de Investigación—Prevalencia de Campylobacter resistente a tetraciclina en pollos de engorde orgánicos durante un ciclo de producción.

En este estudio se investigó la resistencia a la tetraciclina en Campylobacter aislados de pollos de engorde criados de manera orgánica. Se tomaron semanalmente 245 muestras de una granja orgánica de pollos de engorde desde el inicio hasta el final del ciclo de producción y fueron analizadas para determinar la presencia de Campylobacter termofílico. La resistencia a la tetraciclina en estos aislamientos de Campylobacter se determinó mediante el método de dilución en agar, mientras que los patrones de restricción del ADN de cepas susceptibles y resistentes a la tetraciclina fueron determinados mediante electroforesis en campo de pulsaciones. Ninguno de los aislamientos de Campylobacter del periodo correspondiente a la tercera o cuarta semana de producción resultaron resistentes a la tetraciclina, mientras que el 66.7% de los aislamientos a partir de la quinta semana resultaron resistentes a este antibiótico. Aun cuando la prevalencia de la resistencia a tetraciclina alcanzó un 100% durante la sexta y séptima semana de edad, menos del 34% de los aislamientos de la décima semana eran resistentes a este agente antimicrobiano. Adicionalmente, solo13.8% de los aislamientos de Campylobacter provenientes del tracto intestinal de estos pollos de engorde criados de manera orgánica eran resistentes a la tetraciclina. Se detectó la presencia del gen tet(O) en 98.9% de los aislamientos de Campylobacter resistentes a la tetraciclina y las cepas de Campylobacter susceptibles y resistentes a la tetraciclina mostraron patrones diferentes de electroforesis en campo de pulsaciones. Los re

Taradon Luangtongkum, Teresa Y. Morishita, Lori Martin, Irene Choi, Orhan Sahin, and Qijing Zhang "Prevalence of Tetracycline-Resistant Campylobacter in Organic Broilers During a Production Cycle," Avian Diseases 52(3), 487-490, (1 September 2008). https://doi.org/10.1637/8181-112807-ResNote.1
Received: 28 November 2007; Accepted: 1 March 2008; Published: 1 September 2008
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