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1 September 2008 Antimicrobial Susceptibilities, Serogroups, and Molecular Characterization of Avian Pathogenic Escherichia coli Isolates in Japan
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Abstract

In total, 83 avian pathogenic Escherichia coli (APEC) isolates from avian colibacillosis during a period from 2001 to 2006 in Japan were investigated for serogroups, typical virulence factors, antimicrobial susceptibility, and genetic relatedness. The most common serogroup was O78 (30.1%); 80.7% of isolates harbored the iss gene and 55.4% of isolates harbored the tsh gene. Antimicrobial resistance of the isolates was found for ampicillin (77.1%), oxytetracycline (75.9%), kanamycin (36.1%), fradiomycin (33.7%), trimethoprim (25.3%), enrofloxacin (21.7%), and florfenicol (6.0%). Although multiple antimicrobial-resistant phenotypes (three or more antimicrobials) accounted for 54.2% of isolates, no isolate exhibited resistance to all agents tested. The fluoroquinolone-resistant isolates had point mutations in GyrA (Ser83→Leu, Asp87→Asn) and ParC (Ser80→Ile, Glu84→Gly). Of 18 enrofloxacin-resistant E. coli isolates, nine isolates belonged to serotype O78. In PFGE analysis, eight of the nine enrofloxacin-resistant O78 isolates were classified into an identical cluster. This suggests that a specific genotype of fluoroquinolone-resistant O78 APEC may be widely distributed in Japan.

Susceptibilidad antimicrobiana, serogrupos y caracterización molecular de aislamientos de Escherichia coli patógena en Japón.

Un total de 83 aislamientos de Escherichia coli patógena provenientes de casos de colibacilosis aviar en Japón durante los años 2001 al 2006, fueron investigados para determinar su serogrupo, la presencia de factores típicos de virulencia, su susceptibilidad antimicrobiana y su relación genética. El serogrupo mas común fue el O78 (30.1%), el 80.7% de los aislamientos contenían el gen iss y el 54.4% de los aislamientos contenían el gen tsh. Entre los aislamientos se observó resistencia antibacteriana contra la ampicilina (77.1%), oxitetraciclina (75.9%), kanamicina (36.1%), fradiomicina (33.7%), trimetropina (25.3%), enrofloxacina (21.7%) y florfenicol (6%). Aun cuando los fenotipos de resistencia múltiple (tres o mas antibióticos) representaban el 54.2% de los aislamientos, ningún aislamiento mostró resistencia a todos los agentes antimicrobianos evaluados. Los aislamientos resistentes a la fluoroquinolona presentaron mutaciones puntuales en los genes GyrA (Ser83- Leu y Asp87- Asn), así como en el gen ParC (Ser80-Ile, y Glu84-Gly). De 18 aislamientos de E. coli resistentes a la enrofloxacina, nueve aislamientos pertenecían al serotipo O78. En el análisis mediante electroforesis de campo pulsado, ocho de los nueve aislamientos O78 resistentes a enrofloxacina se clasificaron en el mismo grupo. Esto sugiere que un genotipo específico de E. coli patógena del serogrupo O78 resistente a las fluoroquinolonas puede estar ampliamente distribuido en Japón.

Abbreviations: ABPC = ampicillin; APEC = avian pathogenic Escherichia coli; ERFX = enrofloxacin; FFC = florfenicol; FM = fradiomycin; KM = kanamycin; MIC = minimum inhibitory concentration; NCCLS = National Committee for Clinical Laboratory Standards; OTC = oxytetracycline; PCR = polymerase chain reaction; PFGE = pulsed-field gel electrophoresis; QRDR = quinolone-resistant determining region; TMP = trimethoprim; UT = untypeable

Manao Ozawa, Kazuki Harada, Akemi Kojima, Tetsuo Asai, and Toshiya Sameshima "Antimicrobial Susceptibilities, Serogroups, and Molecular Characterization of Avian Pathogenic Escherichia coli Isolates in Japan," Avian Diseases 52(3), 392-397, (1 September 2008). https://doi.org/10.1637/8193-120907-Reg
Received: 10 December 2007; Accepted: 1 March 2008; Published: 1 September 2008
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