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1 September 2008 Limited Susceptibility and Lack of Systemic Infection by an H3N2 Swine Influenza Virus in Intranasally Inoculated Chickens
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Abstract

Chickens were intranasally inoculated with the swine influenza virus (SIV) A/swine/NC/307408/04 (H3N2) (NC/04 SIV) to determine the infectivity of a North American SIV for chickens, as well as the possibility of chicken meat serving as a transmission vehicle for SIV. White leghorn (WL) layer-type chickens were used for initial pathotyping and infectivity tests, and a more comprehensive intranasal pathogenesis study was done with white Plymouth rock (WPR) broiler-type chickens. None of the NC/04 SIV-inoculated WL or WPR chickens displayed clinical signs. Serologic tests showed that the virus was able to infect both intranasally inoculated WL and WPR chickens, but the antibody titers were low, suggesting inefficient replication. Some of the NC/04 SIV-inoculated WL chickens shed low levels of virus, mostly from the alimentary tract, but viral shedding was not detected in NC/04 SIV-inoculated WPR chickens. The comprehensive pathogenesis study demonstrated that the virus did not cause systemic infections in WPR chickens, and feeding breast and thigh meat from the NC/04 SIV-inoculated WPR to WL chickens did not transmit NC/04 SIV.

Abbreviations: AI = avian influenza; AIV = avian influenza virus(es); BHI = brain-heart infusion medium; DPI = days postinoculation; ECE = embryonating chicken egg; EID50 = 50% chicken embryo infective dose; GMT = geometric mean titer; H = hemagglutinin; HI = hemagglutinin inhibition; HPAIV = high pathogenicity avian influenza virus(es); HPNAI = high pathogenicity notifiable avian influenza; LPAI = low pathogenicity avian influenza; LPNAI = low pathogenicity notifiable avian influenza; N = neuraminidase; OIE = World Organization for Animal Health; SIV = swine influenza virus; SPF = specific-pathogen-free; WL = white leghorn; WPR = white Plymouth rock

Nota de Investigación—Susceptibilidad limitada y carencia de infección sistémica en pollos inoculados intranasalmente con un virus H3N2 de influenza porcina.

Para determinar la infectividad en pollos de un virus Norteamericano de influenza porcina, así como la posibilidad de que la carne de pollo sirva como un vehículo de transmisión para el virus de influenza porcina, se inocularon pollos por vía intranasal con un virus H3N2 de influenza porcina designado A/cerdo/NC/307408/04 (por sus siglas en Inglés NC/04SIV). Para las pruebas iniciales de patotipificación e infectividad se utilizaron aves de la línea leghorn blanca y un estudio de patogenicidad más completo se realizó con aves de la línea de engorde (Plymouth rock). Ninguna de las aves leghorn blancas o Plymouth rock inoculadas con el virus NC/04SIV mostraron signos clínicos. Las pruebas serológicas demostraron que el virus fue capaz de infectar por vía intranasal a ambos tipos de aves, sin embargo, los títulos de anticuerpos fueron bajos sugiriendo una replicación ineficiente. Algunas de las aves leghorn blancas diseminaron bajos niveles del virus, predominantemente del tracto alimenticio. En las aves Plymouth rock inoculadas con el virus NC/04SIV no se detectó diseminación viral. El estudio completo de patogénesis demostró que el virus no causó infección sistémica en pollos Plymouth rock y que alimentar carne de pechuga y muslos proveniente de aves leghorn blancas y Plymouth rock inoculadas con el virus NC/04 SIV no transmitió el virus.

Colleen Thomas, Timofey B. Manin, Artem V. Andriyasov, and David E. Swayne "Limited Susceptibility and Lack of Systemic Infection by an H3N2 Swine Influenza Virus in Intranasally Inoculated Chickens," Avian Diseases 52(3), 498-501, (1 September 2008). https://doi.org/10.1637/8210-011408-RESNOTE.1
Received: 16 January 2008; Accepted: 1 April 2008; Published: 1 September 2008
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