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1 September 2008 Sequence Analysis of Four Double-Stranded RNA Genomic Segments Reveals an Orthoreovirus with a Unique Genotype Infecting Psittaciformes
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Abstract

This paper describes the characterization of four double-stranded ribonucleic acid segments, S1, S2, S3, and S4, of a newly identified pathogenic reovirus from parrots. The four segments share a unique 5′ terminus GCUUUUC. The amino-acid sequences of the conserved sigma A and sigma NS proteins show less than 60% sequence similarity, whereas those of the outer capsid proteins sigma B and sigma C have at most 47% sequence similarity to their counterparts in other bird or bat reoviruses. In a phylogenetic analysis of the amino-acid sequences, the proteins coded for by the S1 segment, P10, P17, and sigma C, group with their homologous proteins in other avian reoviruses, whereas the major capsid protein, sigma B, and the nonstructural protein, sigma NS, show more sequence similarity to their bat reoviral counterparts. The phylogenetic relationship of sigma A with the homologous avian and bat sequences is unresolved. The possibility that the parrot reovirus has evolved from an ancestral, more batlike reovirus is discussed. It is proposed to designate this unique virus as PsRV.

Este artículo describe la caracterización de cuatro segmentos de ácido ribonucléico de cadena doble, S1, S2, S3, y S4, pertenecientes a un reovirus de los loros identificado recientemente. Los cuatro segmentos comparten un terminal 5′ único: GCUUUUC. La secuencia de aminoácidos de las proteínas sigma A y la proteína no estructural (NS) muestran menos de 60% de similitud con sus contrapartes en los reovirus de murciélagos y de otros pájaros, mientras las secuencias de las proteínas de la cápsula externa, sigma B y sigma C tienen un máximo de 47% de similitud. En un análisis filogenético de la secuencia de aminoácidos, las proteínas codificadas por el segmento S1, P10, P17 y sigma C, se agruparon con las proteínas homólogas pertenecientes a otros reovirus aviares, mientras que la proteína principal del cápsido, la proteína sigma B y la proteína no estructural sigma NS, muestran similitud en la secuencia a sus contrapartes en los reovirus de murciélago. La relación filogenético de sigma A con las secuencias homólogas en aves y murciélagos no se ha aclarado. Se discute la posibilidad de que el reovirus de los loros haya evolucionado de un reovirus ancestral más parecido a los reovirus de murciélago. Se propone designar a este virus nuevo como PsRV.

Abbreviations: AMV = avian myeloblastosis virus; ARV = avian reovirus; BLAST = Basic Local Alignment Search Tool; BRV = baboon reovirus; DNA = deoxyribonucleic acid; DRV = duck reovirus; EDTA = ethylenediaminetetraacetic acid; ERS = enteric reovirus strain; GRV = goose reovirus; kb = kilobases; MEGA = Molecular Evolutionary Genetics Analysis; MRV = mammalian reovirus; NBV = Nelson Bay virus; NJ = neighbor-joining; NT(s) = nucleotide(s); ORF = open reading frame; PCR = polymerase chain reaction; PRV = Pulau reovirus; PsRV = psittacine reovirus; RNA = ribonucleic acid; RT = reverse transcriptase; TAE = tris-acetate-EDTA; TRV NC = turkey reovirus North Carolina; TVAV = Tvarminne avian virus; USDA = United States Department of Agriculture; USFWS = United States Fish and Wildlife Service; UTR = untranslated region

Análisis de la secuencia de cuatro segmentos genómicos de ARN de cadena doble revela un Orthoreovirus con un genotipo único que infecta psitácidos.

Siwo R. de Kloet "Sequence Analysis of Four Double-Stranded RNA Genomic Segments Reveals an Orthoreovirus with a Unique Genotype Infecting Psittaciformes," Avian Diseases 52(3), 480-486, (1 September 2008). https://doi.org/10.1637/8212-011908-Reg.1
Received: 24 January 2008; Accepted: 1 May 2008; Published: 1 September 2008
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