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1 December 2008 Seroprevalence of Avian Reovirus in Egg-Laying Chicken Flocks in China
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Abstract

In the present study, the epidemiologic status of avian reovirus (ARV) infections was investigated from egg-laying chicken flocks in China with an enzyme-linked immunosorbent assay (ELISA) kit. Because the chickens were not vaccinated against ARV, antibodies were attributed to the infection. Antibodies specific to ARV were found in more than 92% (542/587) of the average positivity and ranged from 30% to 100% in different chicken population. A virus, designated HB06, was isolated from flocks with suspicious ARV infections. Sequence analysis of the S1 gene revealed that strain HB06 was closely related with the most ARVs with less than 2% nucleotide divergence, and the homology was highest with the vaccine strain S1133, with a 98.97% nucleotide identity. The potential significance of vaccination against ARV in egg-laying chicken flocks in China is also discussed.

Abbreviations: ARV = avian reovirus; CAM = chorioallantoic membrane; ELISA = enzyme-linked immunosorbent assay; PBS = phosphate-buffered saline; PCR = polymerase chain reaction; RT = reverse transcription; S/P = sample-to-positive ratio; SPF = specific-pathogen-free; TMB = 1,2,4,5-tetramethoxybenzene

Nota de Investigación—Prevalencia serológica de reovirus aviares en lotes de ponedoras comerciales en China.

Se investigó la situación epidemiológica de las infecciones por reovirus aviares en lotes de ponedoras comerciales de China. Se utilizó el inmunoensayo con enzimas asociadas (ELISA) para evaluar la presencia de anticuerpos en el suero de las aves. Debido a que las aves no son vacunadas contra reovirus, la presencia de anticuerpos fue atribuida a infección de campo. Se encontraron anticuerpos específicos contra reovirus en más del 92% (542/587) de la positividad promedia que varió del 30% al 100% en las diferentes poblaciones de aves. Se aisló un virus, designado como HB06, de lotes sospechosos de infecciones por reovirus. El análisis de la secuencia del gene S1 mostró que la cepa HB06 estaba estrechamente relacionada con la mayoría de los reovirus aviares, con menos del 2% de divergencia en secuencia de nucleótidos, y la homología fue la mayor con la cepa vacunal S1133, con una identidad para la secuencia de nucleótidos del 98.97%. Se discute la significancia potencial de la vacunación contra reovirus aviares en lotes de ponedoras comerciales de China.

Juan Pu, Xingli Liu, Youchun Guo, Yu Cao, Jixun Zhao, and Guozhong Zhang "Seroprevalence of Avian Reovirus in Egg-Laying Chicken Flocks in China," Avian Diseases 52(4), 675-679, (1 December 2008). https://doi.org/10.1637/8300-040108-Reg.1
Received: 17 April 2008; Accepted: 1 July 2008; Published: 1 December 2008
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