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1 December 2008 Prevalence of Antibodies to Different Avian Paramyxoviruses in Commercial Poultry in the United States
Ashwini Warke, Lauren Appleby, Egbert Mundt
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Abstract

Avian paramyxoviruses (APMVs) other than Newcastle disease virus have been isolated from feral avian species and have the potential to cause clinical disease. The objective of this study was to investigate the APMV antibody prevalence in commercial poultry settings. Sera from 100 commercial, layer-type and broiler-type (broiler–breeder and broiler) chicken flocks were analyzed on a random basis. Pooled serum samples from each flock were tested for the presence of antibodies to APMV-1, -2, -3, -4, -6, -7, -8, and -9 by hemagluttination inhibition (HI) test. Reactions with HI titers ≥1:64 were shown by APMV-1 (71%), APMV-2 (15%), APMV-3 (35%), APMV-4 (26%), APMV-6 (45%), APMV-7 (27%), APMV-8 (31%), and APMV-9 (48%). In the presence of a high HI titer for APMV-1 (1024), we obtained positive HI titers for most of the other APMV subtypes, thus implicating that sensitive techniques need to be developed to detect the prevalence of these subtypes in commercial poultry.

Abbreviations: APMVs = avian paramyxoviruses; cRBC = chicken red blood cells; HA = hemagglutination; HI = hemagglutination inhibition; NDV = Newcastle disease virus; NVSL = National Veterinary Services Laboratory; PBS = phosphate-buffered saline; PDRC = Poultry Diagnostic and Research Center; RT = room temperature

Nota de Investigación—Prevalencia de anticuerpos contra diferentes paramyxovirus aviares en la avicultura comercial de los Estados Unidos.

Los paramyxovirus aviares diferentes al virus de Newcastle se han aislado a partir de especies aviares salvajes y tienen el potencial de causar enfermedad clínica. El objetivo de este estudio fue investigar la prevalencia de anticuerpos contra los paramyxovirus aviares en la avicultura comercial de Estados Unidos. Sueros tomados de 100 lotes de aves comerciales incluyendo ponedoras, reproductoras de engorde y pollos fueron analizados al azar. Las muestras de suero de cada lote fueron mezcladas en una sóla muestra y analizadas por medio de la prueba de inhibición de la hemoaglutinación para la presencia de anticuerpos contra paramyxovirus de los subtipos 1, 2, 3, 4, 6, 7, 8 y 9. Se encontraron reacciones con títulos mayores de 64 para paramyxovirus 1 (71%), paramyxovirus 2 (15%), paramyxovirus 3 (35%), paramyxovirus 4 (26%), paramyxovirus 6 (45%), paramyxovirus 7 (27%), paramyxovirus 8 (31%), y paramyxovirus 9 (48%). Se obtuvieron títulos inhibidores de la hemoaglutinación positivos para la mayoría de los otros subtipos de paramyxovirus en presencia de un alto título para paramyxovirus del subgrupo 1 (≥1,024), implicando que se deben desarrollar técnicas sensibles para detectar la prevalencia de estos subtipos en la avicultura comercial.

Ashwini Warke, Lauren Appleby, and Egbert Mundt "Prevalence of Antibodies to Different Avian Paramyxoviruses in Commercial Poultry in the United States," Avian Diseases 52(4), 694-697, (1 December 2008). https://doi.org/10.1637/8390-070308-RESNOTE.1
Received: 7 July 2008; Accepted: 1 August 2008; Published: 1 December 2008
KEYWORDS
avian paramyxoviruses
commercial poultry
serosurvey
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