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1 March 2010 Evaluation of Neuraminidase Antigen Based Competitive Enzyme-Linked Immunosorbent Assay in Chickens Vaccinated with Avian Influenza Inactivated Vaccine
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Abstract

A competitive enzyme-linked immunosorbent assay (c-ELISA) was developed as a serologic diagnostic tool to detect antibodies against NA subtype 3 of avian influenza virus (AIV). The NA antigen used in this c-ELISA was obtained by pronase treatment of allantoic fluid of specific-pathogen-free (SPF) eggs infected with AIV. The NA specific monoclonal antibodies were produced from purified NA. The N3 c-ELISA was carried out on serum samples collected from both SPF chickens and commercial layers to confirm whether the N3 c-ELISA was capable of detecting specific N3 antibodies. The positive cutoff percentage inhibition value was 6.13%. The sensitivity and specificity of the N3 c-ELISA were 83.7% and 95.6%, respectively, which indicated that N3 c-ELISA can detect the antibodies from SPF chickens or commercial chickens vaccinated with H9N3 subtype of AIV.

Jong-Nyeo Kim, Seong-Hwan Byun, Shien-Young Kang, and In-Pil Mo "Evaluation of Neuraminidase Antigen Based Competitive Enzyme-Linked Immunosorbent Assay in Chickens Vaccinated with Avian Influenza Inactivated Vaccine," Avian Diseases 54(s1), 682-685, (1 March 2010). https://doi.org/10.1637/8747-033009-ResNote.1
Received: 30 March 2009; Accepted: 1 October 2009; Published: 1 March 2010
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