Antiluteolytic actions of bovine interferon-tau (bIFN-τ) require suppression of prostaglandin F2α (PGF2α) production. Our objective was to test whether bIFN-τ could block PGF2α production and synthesis of phospholipase A2 (PLA2) and cyclooxygenase-2 (COX-2) enzymes induced by a protein kinase C (PKC) stimulator (phorbol 12,13 dibutyrate; PDBu). Bovine endometrial epithelial (BEND) cells were treated with PDBu in the presence or absence of bIFN-τ. Medium samples were analyzed for concentrations of PGF2α, whole-cell extracts were analyzed for abundance of PLA2 and COX-2 by immunoblotting, and RNA extracts were examined for steady-state levels of COX-2 mRNA by Northern blotting. The PDBu stimulated production of PGF2α between 3 and 12 h, levels of COX-2 mRNA by 3 h and protein expression of COX-2 and PLA2 by 6 and 12 h, respectively. Added concomitantly with PDBu, bIFN-τ suppressed PGF2α production, steady-state levels of COX-2 mRNA, and expression of COX-2 and PLA2 proteins. Added after a 3-h stimulation with PDBu alone, bIFN-τ suppressed PGF2α production after 1 h. Bovine IFN-τ inhibited intracellular mechanisms responsible for PGF2α production in BEND cells, and this could be through both cytosolic and nuclear actions.
How to translate text using browser tools
1 August 2000
Interferon-τ Modulates Phorbol Ester-Induced Production of Prostaglandin and Expression of Cyclooxygenase-2 and Phospholipase-A2 from Bovine Endometrial Cells
Mario Binelli,
Aydin Guzeloglu,
Lokenga Badinga,
Daniel R. Arnold,
Jean Sirois,
Thomas R. Hansen,
William W. Thatcher
ACCESS THE FULL ARTICLE
conceptus
hormone action
kinases
pregnancy
uterus