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1 January 2001 Direct Exposure of Chromosomes to Nonactivated Ovum Cytoplasm Is Effective for Bovine Somatic Cell Nucleus Reprogramming
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Abstract

We examined the in vitro developmental potential of nonactivated and activated enucleated ova receiving cumulus cells at various stages of the cell cycle. Eleven to 29% of activated ova receiving donor cells stopped developing at the 8-cell stage but 21% to 50% of nonactivated ova receiving donor cells at either the G0, G1, G2, or M phase, or cycling cells developed into blastocysts. One normal calf was born after transferring five blastocysts that had developed from ova receiving donor cells at the M phase. The present study demonstrated that direct exposure of donor chromosomes to nonactivated ovum cytoplasm is effective for somatic cell nucleus reprogramming, and activated ovum cytoplasm does not reprogram the nucleus.

Tetsuya Tani, Yoko Kato, and Yukio Tsunoda "Direct Exposure of Chromosomes to Nonactivated Ovum Cytoplasm Is Effective for Bovine Somatic Cell Nucleus Reprogramming," Biology of Reproduction 64(1), 324-330, (1 January 2001). https://doi.org/10.1095/biolreprod64.1.324
Received: 31 May 2000; Accepted: 5 September 2000; Published: 1 January 2001
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