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1 January 2001 Rat Seminiferous Epithelium Contains a Unique Junction (Ectoplasmic Specialization) with Signaling Properties Both of Cell/Cell and Cell/Matrix Junctions
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Abstract

The seminiferous epithelium contains unique actin related cell-cell junctions, termed ectoplasmic specializations (ESs). Turnover of these junctions is fundamental to sperm release and to movement of spermatocytes from basal to adluminal compartments of the epithelium during spermatogenesis. In this study we report several novel observations related to the spatial and temporal distribution of integrin-related signaling molecules at ESs. We confirm the presence of β1-integrin at these sites and further demonstrate co-localization of integrin linked kinase (ILK). β1-Integrin and ILK were shown by immunoprecipitation to associate in whole cell lysates of seminiferous epithelium. This observation provides the first evidence for a direct β1-integrin/ILK interaction in noncultured epithelium. Pan-cadherin and β-catenin antibodies did not react at ESs. Rather, antibodies reacted with desmosome-like junctions that are present both at basal junctional complexes between Sertoli cells and at sites of attachment to spermatogenic cells. Focal adhesion kinase (FAK), a known integrin-associated molecule, did not codistribute with β1-integrins and did not associate with these adhesion molecules in immunoprecipitation studies. Although FAK was expressed in the epithelium, it appeared to be limited to the cytoplasm of early spermatogenic cells. Significantly, polyclonal antibodies against phosphotyrosine-containing residues reacted strongly at ESs, with highest levels detected during sperm release and turnover of basal junction complexes. Our observations indicate that ESs share cell signaling features both of cell-cell junctions and of cell-extracellular matrix junctions.

David J. Mulholland, Shoukat Dedhar, and A. Wayne Vogl "Rat Seminiferous Epithelium Contains a Unique Junction (Ectoplasmic Specialization) with Signaling Properties Both of Cell/Cell and Cell/Matrix Junctions," Biology of Reproduction 64(1), 396-407, (1 January 2001). https://doi.org/10.1095/biolreprod64.1.396
Received: 13 June 2000; Accepted: 5 September 2000; Published: 1 January 2001
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