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1 June 2001 Endothelin-1 Mediates Prostaglandin F-Induced Luteal Regression in the Ewe
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Abstract

A diversified series of experiments was conducted to determine the potential role of endothelin-1 (ET-1) in ovine luteal function. Endothelin-1 inhibited basal and LH-stimulated progesterone production by dispersed ovine luteal cells during a 2-h incubation. This inhibition was removed when cells were preincubated with cyclo-d-Asp-Pro-d-Val-Leu-d-Trp (BQ123), a highly specific endothelin ETA receptor antagonist. Administration of a luteolytic dose of prostaglandin F (PGF) rapidly stimulated gene expression for ET-1 in ovine corpora lutea (CL) collected at midcycle. Intraluteal administration of a single dose of BQ123 to ewes on Day 8 or 9 of the estrous cycle mitigated the luteolytic effect of PGF. Intramuscular administration of 100 μg ET-1 to ewes at midcycle reduced plasma progesterone concentrations for the remainder of the estrous cycle. Following pretreatment with a subluteolytic dose of PGF, i.m. administration of 100 μg ET-1 caused a rapid decline in plasma progesterone and shortened the length of the estrous cycle. These data complement and extend previously published reports in the bovine CL and are the strongest evidence presented to date in support of a role for ET-1 in PGF-mediated luteal function in domestic ruminants.

S. T. Hinckley and R. A. Milvae "Endothelin-1 Mediates Prostaglandin F-Induced Luteal Regression in the Ewe," Biology of Reproduction 64(6), 1619-1623, (1 June 2001). https://doi.org/10.1095/biolreprod64.6.1619
Received: 5 June 2000; Accepted: 1 January 2001; Published: 1 June 2001
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