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1 June 2001 Stimulation by N6-Cyclopentyladenosine of A1 Adenosine Receptors, Coupled to Gαi2 Protein Subunit, Has a Capacitative Effect on Human Spermatozoa
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Abstract

The effects of selective A1 receptor agonist on human spermatozoa were examined to verify physiological responses and to investigate the signal transduction pathway. N6-Cyclopentyladenosine on uncapacitated spermatozoa did not induce spontaneous acrosome reaction after 5 h capacitation, whereas the number of capacitated spermatozoa, assessed by lysophosphatidylcholine-induced acrosome reaction with Pisum sativum agglutinin staining, was significantly increased. N6-Cyclopentyladenosine was also added to capacitated human spermatozoa to find out whether the agonist could induce the acrosome reaction. Results, although statistically significant, could not be considered biologically significant. A1-Mediated capacitation was followed by the increase of tyrosine phosphorylation of a protein subset ranging between Mr = 200 000 and 30 000. Stimulation of A1 receptor with the selective agonist elicited an agonist-induced inositol phospholipid hydrolysis leading to a transient rise of inositol triphosphate (IP3). This increase was not induced by A1 receptor antagonist and was blocked by phospholipase C inhibitor. Coimmunoprecipitation experiments showed that the A1 receptor is coupled to Gαi2 subunit suggesting that the activation of phospholipase C is mediated by βγ subunits. In conclusion, the A1 adenosine receptor in human spermatozoa is coupled to Gαi2, signals via IP3, and affects the capacitative status of ejaculated spermatozoa.

Cinzia Allegrucci, Lavinia Liguori, and Alba Minelli "Stimulation by N6-Cyclopentyladenosine of A1 Adenosine Receptors, Coupled to Gαi2 Protein Subunit, Has a Capacitative Effect on Human Spermatozoa," Biology of Reproduction 64(6), 1653-1659, (1 June 2001). https://doi.org/10.1095/biolreprod64.6.1653
Received: 12 October 2000; Accepted: 1 January 2001; Published: 1 June 2001
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