Translator Disclaimer
1 June 2001 Autocrine/Paracrine Action of Oxytocin in Pig Endometrium
Author Affiliations +
Abstract

Luminal epithelial cells of porcine endometrium are unresponsive to oxytocin (OT) in vitro although they express the greatest quantity of OT and receptors for OT in vivo. Therefore, the objective of this study was to determine if oxytocin acted in an autocrine manner on luminal epithelial cells to stimulate prostaglandin (PG)F secretion. Treatment of endometrial explants or enriched luminal epithelial cells with OT antagonist L-366,948 decreased (P < 0.05) basal secretion of PGF. Oxytocin increased (P < 0.01) PGF secretion from luminal epithelial cells that were pretreated with 1:5000 or 1:500 OT antiserum for 3 h to immunoneutralize endogenously secreted OT. However, OT only increased (P < 0.05) PGF secretion from glandular epithelial cells when pretreated with 1:500 OT antiserum. Pretreatment with OT antiserum did not alter the ability of OT to induce PGF secretion from stromal cells. Medium conditioned by culture of luminal epithelial cells stimulated (P < 0.05) phospholipase C activity in stromal cells, indicative of the presence of bioactive OT. Oxytocin was secreted by luminal epithelial cells and 33% was released from the apical surface. These results indicate that luminal epithelial cells secrete OT that acts in an autocrine and/or paracrine manner in pig endometrium to stimulate PGF secretion.

Jianbo Hu, Tenneille E. Ludwig, Ugur Salli, Fredrick Stormshak, and Mark A. Mirando "Autocrine/Paracrine Action of Oxytocin in Pig Endometrium," Biology of Reproduction 64(6), 1682-1688, (1 June 2001). https://doi.org/10.1095/biolreprod64.6.1682
Received: 7 November 2000; Accepted: 1 January 2001; Published: 1 June 2001
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top