Translator Disclaimer
1 April 2002 Analysis of the Roles of RGD-Binding Integrins, α49 Integrins, α6 Integrins, and CD9 in the Interaction of the Fertilin β (ADAM2) Disintegrin Domain with the Mouse Egg Membrane
Author Affiliations +
Abstract

Fertilin β (also known as ADAM2), a mammalian sperm protein that mediates gamete cell adhesion during fertilization, is a member of the ADAM protein family whose members have disintegrin domains with homology to integrin ligands found in snake venoms. Fertilin β utilizes an ECD sequence within its disintegrin domain to interact with the egg plasma membrane; the Asp is especially critical. Based on what is known about different integrin subfamilies and their ligands, we sought to characterize fertilin β binding sites on mouse eggs, focusing on integrin subfamilies that recognize short peptide sequences that include an Asp residue: the α58vIIb or RGD-binding subfamily (α5β1, α8β1, αVβ1, αVβ3, αVβ5, αVβ6, αVβ8, and αIIbβ3) and the α49 subfamily (α4β1, α9β1, and α4β7). We tested peptide sequences known to perturb interactions mediated by these integrins in two different assays for fertilin β binding. Peptides with the sequence MLDG, which perturb α49 integrin-mediated interactions, significantly inhibit fertilin β binding to eggs, which suggests a role for a member of this integrin subfamily as a fertilin β receptor. RGD peptides, which perturb α58vIIb integrin-mediated interactions, have partial inhibitory activity. The anti-α6 antibody GoH3 has little or no inhibitory activity. An antibody to the integrin-associated tetraspanin protein CD9 inhibits the binding of a multivalent presentation of fertilin β (immobilized on beads) but not soluble fertilin β, which we speculate has implications for the role of CD9 in the strengthening of fertilin β-mediated cell adhesion but not in initial ligand binding.

Xiaoling Zhu and Janice P. Evans "Analysis of the Roles of RGD-Binding Integrins, α49 Integrins, α6 Integrins, and CD9 in the Interaction of the Fertilin β (ADAM2) Disintegrin Domain with the Mouse Egg Membrane," Biology of Reproduction 66(4), 1193-1202, (1 April 2002). https://doi.org/10.1095/biolreprod66.4.1193
Received: 4 October 2001; Accepted: 1 November 2001; Published: 1 April 2002
JOURNAL ARTICLE
10 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top