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1 June 2002 Involvement of Na -HCO3 Cotransporter in Mediating Cyclic Adenosine 3′,5′-Monophosphate-Dependent HCO3 Secretion by Mouse Endometrial Epithelium
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Abstract

The present study investigated the involvement of Na -HCO3 cotransporter in mediating cAMP-stimulated HCO3 secretion across the cultured mouse endometrial epithelium using the short-circuit current (ISC) technique and intracellular pH measurement. Forskolin stimulated a rise in the ISC, 55.6% and 52.1% of which could be reduced by the removal of extracellular Cl or by eliminating the contribution of Cl secretion by bumetanide, an inhibitor of Na -K -2Cl cotransporter, respectively. More than 80% reduction in the forskolin-induced ISC was obtained when both Cl and HCO3 in the bath were removed or in HCO3-free solution with bumetanide, indicating that the ISC depended on both Cl and HCO3. The presence of the Na channel-blocker amiloride in the apical solution did not reduce the forskolin-induced ISC; however, the ISC could be abolished by removing Na from the bathing solution, suggesting that the Cl- and HCO3-dependent ISC was also dependent on basolateral Na . The forskolin-stimulated ISC could be reduced 43.6% by removal of HCO3 and 47.9% by a Na -HCO3-cotransporter inhibitor, dihydrogen-4,4′-didsothiocyanostilbene-2,2′-disulfonic acid (H2DIDS). The inhibitory effect of H2DIDS was observed in Cl-free solution, but not when HCO3 was removed, thus confirming its effect on HCO3-dependent transport. Intracellular pH measurements demonstrated that the recovery from cellular acidification depended on the presence of both basolateral Na and HCO3, further indicating the involvement of Na -HCO3 cotransporter. Reverse transcription-polymerase chain reaction experiments confirmed the expression of Na -HCO3 cotransporter in the mouse endometrium. The results suggest that basolaterally located Na -HCO3 cotransporter is involved in mediating cAMP-stimulated HCO3 secretion across the mouse endometrial epithelium.

X. F. Wang, M. K. Yu, K. M. Leung, C. Y. Yip, W. H. Ko, C. Q. Liu, and H. C. Chan "Involvement of Na -HCO3 Cotransporter in Mediating Cyclic Adenosine 3′,5′-Monophosphate-Dependent HCO3 Secretion by Mouse Endometrial Epithelium," Biology of Reproduction 66(6), 1846-1852, (1 June 2002). https://doi.org/10.1095/biolreprod66.6.1846
Received: 17 September 2001; Accepted: 1 January 2002; Published: 1 June 2002
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