Translator Disclaimer
1 January 2003 Regulated Expression and Ultrastructural Localization of Galectin-1, a Proapoptotic β-Galactoside-Binding Lectin, During Spermatogenesis in Rat Testis
Author Affiliations +
Abstract

Galectin-1, a highly conserved β-galactoside-binding protein, induces apoptosis of activated T cells and suppresses the development of autoimmunity and chronic inflammation. To gain insight regarding the potential role of galectin-1 as a novel mechanism of immune privilege, we investigated expression and ultrastructural localization of galectin-1 in rat testis. Galectin-1 expression was assessed by Western blot analysis and immunocytochemical localization in testes obtained from rats aged from 9 to 60 days. Expression of this carbohydrate-binding protein was developmentally regulated, and its immunolabeling exhibited a stage-specific pattern throughout the spermatogenic process. Immunogold staining using the anti-galectin-1 antibody revealed the typical Sertoli cell profile in the seminiferous epithelium, mainly at stages X–II. During spermiation (stages VI–VIII), a strong labeling was observed at the luminal pole of seminiferous epithelium, localized on apical stalks of Sertoli cells, on heads of mature spermatids, and on bodies of residual cytoplasm. Moreover, spermatozoa released into the lumen showed a strong immunostaining. Following spermiation (stage VIII), galectin-1 expression was restored at the basal portion of Sertoli cells and progressively spread out through the whole cells as differentiation of germinal cells proceeded. Immunoelectron microscopy confirmed distribution of galectin-1 in nuclei and cytoplasmic projections of Sertoli cells and on heads and tails of late spermatids and residual bodies. Surface localization of galectin-1 was evidenced in spermatozoa from caput epididymis. Thus, the regulated expression of galectin-1 during the spermatogenic cycle suggests a novel role for this immunosuppressive lectin in reproductive biology.

Luis Dettin, Natalia Rubinstein, Agustín Aoki, Gabriel A. Rabinovich, and Cristina A. Maldonado "Regulated Expression and Ultrastructural Localization of Galectin-1, a Proapoptotic β-Galactoside-Binding Lectin, During Spermatogenesis in Rat Testis 1," Biology of Reproduction 68(1), (1 January 2003). https://doi.org/10.1095/biolreprod.102.006361
Received: 11 April 2002; Accepted: 1 July 2002; Published: 1 January 2003
JOURNAL ARTICLE
9 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top