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1 February 2003 Temporally Controlled Site-Specific Mutagenesis in the Germ Cell Lineage of the Mouse Testis
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Abstract

We have obtained a PrP-Cre-ERT transgenic mouse line (28.8) that selectively expresses in testis the tamoxifen-inducible Cre-ERT recombinase under the control of a mouse Prion protein (PrP) promoter-containing genomic fragment. Cre-ERT is expressed in spermatogonia and spermatocytes, but not in Sertoli and Leydig cells. We also established reporter PrP-L-EGFP-L transgenic mice harboring a LoxP-flanked enhanced green fluorescent protein (EGFP) Cre reporter cassette under the control of the same PrP promoter-containing genomic fragment that exhibits prominent EGFP expression in brain and testis. Using the PrP-L-EGFP-L as well as other Cre-reporter mice, we demonstrate that tamoxifen administration efficiently and selectively induces Cre-mediated recombination in the germ cell lineage. The established PrP-Cre-ERT line should provide a valuable tool for studying functions of germ cell-expressed genes involved in spermatogenesis.

Philipp Weber, Michael Schuler, Christelle Gérard, Manuel Mark, Daniel Metzger, and Pierre Chambon "Temporally Controlled Site-Specific Mutagenesis in the Germ Cell Lineage of the Mouse Testis," Biology of Reproduction 68(2), 553-559, (1 February 2003). https://doi.org/10.1095/biolreprod.102.005801
Received: 25 March 2002; Accepted: 1 August 2002; Published: 1 February 2003
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