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1 March 2003 Contraction of Cultured Human Uterine Smooth Muscle Cells after Stimulation with Endothelin-1
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Abstract

To our knowledge, the problem of how to maintain isolated smooth cells in a “contractile” phenotypic state without deviation after subculturing has yet to be resolved. The present study characterized the in vitro contractile response of human uterine smooth muscle cell to endothelin-1, which induces contractions in isolated uterine strips. Contractile effects were qualitatively investigated using silicone rubber substrata. Endothelin-1 was able to distort and reduce the wrinkles in the silicone surface. Contractions were also quantified by measuring the resulting change in the collagen lattice area. Endothelin-1 significantly increased the contractile response in a dose-dependent manner by selectively activating endothelin A receptors. When myometrial cells were cultured within collagen lattices, a microfilament-disrupting agent, cytochalasin B, abolished contractions, and no change was observed in smooth muscle α-actin immunostaining. Taken together, these observations show that the uterine smooth muscle cells are contractile and respond appropriately to a potent uterotonic agent. Based on these findings, a cultured uterine smooth muscle cell model, which could be used to elucidate the mechanisms controlling uterine activity, is proposed.

Emmanuelle Dallot, Marcel Pouchelet, Nelly Gouhier, Dominique Cabrol, Françoise Ferré, and Michelle Breuiller-Fouché "Contraction of Cultured Human Uterine Smooth Muscle Cells after Stimulation with Endothelin-1," Biology of Reproduction 68(3), 937-942, (1 March 2003). https://doi.org/10.1095/biolreprod.102.008367
Received: 13 June 2002; Accepted: 1 September 2002; Published: 1 March 2003
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